🔬 Cell Culture: culture of mammalian cancer cell lines (human, mouse, rat, hamster/CHO), bacterial culture, aseptic technique, BSCs, bioreactor operation, banking, cryopreservation, mycoplasma testing, cell counting and viability, media optimization, and karyotyping.
🧬 Molecular Biology: bacterial transformations, DNA extraction and purification, cloning, RT-qPCR, ELISA, protein purification, gel electrophoresis (agarose/SDS-PAGE), Bradford assays, spectrophotometry.
💻 Laboratory Instrumentation: Tecan Fluent, Beckman Coulter Biomek i7, Hamilton Star, Hamilton Verso, Terumo Quantum Cell Expansion System, Vi-CELL, NanoEntek Eve, Labcyte Echo, Bio-Rad Opticon 2 and Chromo4 Systems, Roche cobas 4800 system, DNASTAR Lasergene, DNA Strider, BioTek, NanoDrop, plate readers.
📝 Data Management: LIMS, Electronic Lab Notebooks, Microsoft Office, Google Suite.
💡 Experimental and Process Expertise: experimental design, process development, workflow optimization, troubleshooting, data analysis, SOP authorship and implementation.
🥼 Other: cGMP/GLP/GDP regulatory compliance, small molecule handling, sample management (cells, compounds, clinical research), food science, food safety, cross-functional collaboration, supply chain coordination, mentoring.